주름 미백 시험법

소개글

주름과 미백에 관련 된 세포 시험범

목차

1. Anti-wrinkle
(1)Skin aging
(2) Method
(3) Paper

2. Whitening
(1) Dyspigmentation
(2) Method
(3) Paper

3. References

본문내용

Skin aging
- 보통 20세를 전후로 시작
특징 : 두꺼운 각질층 형성, 피부색의 변화, 탄력감소로 인한 주름 생성
요인 : 생리적인 요인, 환경적인 요인, 외부환경적, 생활환경적, 영양학적 요인
내인성 노화(intrinsic aging)
-나이가 들면서 생기는 자연적인 노화
-콜라겐 기질 감소
광노화(photo aging)
-외부환경에 기인하는 것
-콜라겐 파괴

< 중략 >

Melanin content
-멜라닌 합성 억제 정도 측정
Cell seeding (1X105cells/ml) > Treatment > Cell harvest (4℃, 10000rpm, 15min centrifuge)
> Pellet 남김 > 용해 (Pellet+NaOH solution 60℃, 1hr ) > 흡광도 측정

참고 자료

1. Frederick MR, Ruth As, John BW. Effects of coenzyme Q10 treatment on antioxidant pathway in normal and streptozotocin-induced diabetic rats. J Biochem Mol Toxicol 2001; 15:41-46
2. Park SY, Mun KC, Kim BC, Lee KS. The effects of coenzyme Q10, kinetin, and thioctic acid on ultraviolet B irradiated cultured human skin fibroblasts. Korean J Dermatol 2004;42(7):839-845
3. Park SY, Lee KJ, Yi TH. Effect of buddleja officinalis maxim. On melanin biosynthesis and anti-oxidative activity. The Journal of beauty & Trichology 5(1), 00~00(2008)
4. Yagi A., Kanbara T. and Morinobu N. The effect of tyrosinase inhibition for Aloe. Planta Med. 1986; 3981:517-519.
Jeong JH, Wee JJ, Shin JY, Cho JH and Jung DH. Antioxidantive effect of crude saponin fraction prepared from culture product of 5. basidiomycota cultured with fresh ginseng as substrate. Korean J. Food Sci. Technol 2005; 37:67-72