보건영양학 및 실험 <쥐보고서>

소개글

쥐 보고서,쥐의 희생, GPT, 혈중콜레스테롤, 혈중지질

목차

Ⅰ. 이론적 배경 ······················································································································································ 3
1. 1 실험 쥐의 종류 및 특성·························································································································· 3
1. 2 동물실험 ························································································································································ 3
1. 3 녹차 ································································································································································· 3
1. 4 청국장 ····························································································································································· 4
1. 5 지질검사 ························································································································································ 5
1. 5. 1 Triglyceride (TG) ······························································································································ 5
1. 5. 2 Total Cholesterol (TC) ·················································································································· 5
1. 6 GPT ·································································································································································· 5
1. 7 HDL, LDL ······················································································································································· 5

Ⅱ. 실험재료 및 방법 ··········································································································································· 6
2. 1 동물사육방법 ··············································································································································· 6
2. 1. 1 실험에 사용한 동물 ························································································································· 6
2. 1. 2 사육환경 ················································································································································ 6
2. 1. 3 식이조제방법 ······································································································································· 6
2. 2 결과분석방법 ··············································································································································· 9
2. 2. 1 Food intake ········································································································································· 9
2. 2. 2 Body weight ········································································································································ 9
2. 2. 3 쥐 희생과정 ········································································································································ 10
2. 2. 4 혈청분리 (혈청분리) ······················································································································· 12
2. 2. 5 Triglyceride (TG) ······························································································································ 12
2. 2. 6 Total Cholesterol (TC) ··················································································································· 13
2. 2. 7 GPT ························································································································································· 14
2. 2. 8 HDL ························································································································································· 15

Ⅲ. 결과 및 고찰 ··················································································································································· 17
3. 1 동물사육 일지 ············································································································································ 17
3. 2 분석결과 ······················································································································································· 30
3. 2. 1 Body weight ······································································································································ 30
3. 2. 2 식이섭취효율(FER) ··························································································································· 31
3. 2. 3 Triglyceride (TG) ······························································································································ 33
3. 2. 4 Total Cholesterol (TC) ·················································································································· 35
3. 2. 5 GPT ························································································································································ 37
3. 2. 6 HDL ······················································································································································· 39

Ⅳ. 요약 및 결론 ·················································································································································· 42

Ⅴ. 느낀점 ······························································································································································· 43

Ⅵ. 참고문헌 ···························································································································································· 47

본문내용

Ⅰ. 이론적 배경

1. 1 실험쥐의 종류 및 특성
주로 생물학을 실험 하는 사람들이 사용하는 쥐의 일종으로 실험하는 사람들은 주로 SD-rat이라 부른다. SD-rat는 독성화과 약물학을 포함하는 생의학연구의 거의 모든 분야에서 일반적인 목적으로의 연구 모델로써 폭넓게 사용되고 있다. 샘타코는 타코닉으로부터 1998년 처음 도입하였고 타코닉 IBU와 함께 매년 종축을 혼합, 주기적 교체하였다. 카토닉은 1970년에 NIH 동물 유전자원 센터로부터 처음 도입하였고, NIH동물 유전자원 센터의 SD는 1945년 Sprague-Dawley, Inc로부터 outbred closed colony로써 유래된 것이다.

1. 2 동물실험
의학·생물학 연구의 한 방법으로 동물실험을 체계화 한 것은 19세기 프랑스의 클로드 베르나르이다. 동물실험은 생물의 기능해명에 크게 기여했으나, 한편으로는 그 잔혹함 때문에 의학에 반드시 필요한가 하는 의문이 제기되었다. 동물실험 반대운동은 베르나르의 가족과 조수들로부터 시작되었다. 영국에서는 1876년 동물학대방지법이 제정되었으나, 그 후로도 동물실험은 점점 더 대규모화되어 현재 세계에서 연간 2억-3억 마리가 사용되는 것으로 추정되었다. 1970년부터 미국, 유럽에서 동물복지라는 관점 아래 실험의 과학적 타당성에 의문을 갖고 동물실험 반대 시민운동이 일어났다. 이에 유럽회의는 1985년 동물실험을 5년 이내에 전폐하라는 권고를 채택하였다. 또 이탈리아의 남치롤 주 리히텐슈타인에서는 동물실험 전폐법이 제정되었다. 현재 토끼 눈 점막의 약물 자극성을 보는 트레즈 테스트와 반수의 동물이 사망하는 양을 찾아내는 LD 50테스트는 의학상 전혀 도움이 되지 않고 동물만 학대할 뿐이라 하여 세계적으로 폐지하려는 움직임이 강해지고 있다. 그리고 동물실험 외의 방법으로 대체(Replacement), 사용 동물수의 감축(Reduction), 마취 등에 의한 고통완화(Refinement)라는 3R 를 추구하는 방법을 세계 연구자들이 공통으로 인식하게끔 되었다. 대체법 개발은 동물복지 관점만이 아니라 세포배양 등 신뢰할 수 있는 저렴한 방법을 찾자는 동기도 작용한 것으로 알려 졌다.

참고 자료

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