미생물 실험 결과 report Cultivation of Aerobic Microbes
- 최초 등록일
- 2013.04.02
- 최종 저작일
- 2012.04
- 6페이지/
한컴오피스
- 가격 1,000원
소개글
미생물 실험 결과 레포트입니다~
실험 과정, 결과, 디스커션 상세합니다
레포트 점수 A 받았습니다
목차
1 Purpose
2 Materials
3 Procedure
4 Observation & Data
5 Discusison
6 Question
7 Reference
본문내용
Procedure
[Pre - Cultivation]
1. Prepare the materials of the experiment to the clean bench.
2. Pour the MRS broth(3~5㎖) which is in the e-tube into the test tube.
3. Take 1 colony of the cell 1 plate by using a tip. (before and after, do flaming burning)
4. Put the tip into the test tube and mark the test tube.
5. Repeat the procedure 1 ~ 3 to pre - cultivation to get the medium of cell 2.
6. Put test tubes into the shaking incubator and do overnight cultivation.
⇒ Condition : 30 ℃, 200 rpm.
[Main Cultivation]
1. Collect 1 ㎖ of the pre - cultivation medium which is in the test tube and vortexing it.
2. Put the pre - cultivation medium(seed) to the main cultivation medium.
⇒ the amount of the main cultivation medium in the 500 ㎖ flask : 50 ㎖
3. Shake the flask and collect 1 ㎖ of the mixed medium immediately and put it to the e - tube.
4. Vortex 1 ㎖ of the medium which in the e - tube and move it to the cuvette.
5. Measure the optical density(O.D) of the medium.
참고 자료
논문명/저자명 : 형질전환법에 의한 Lactobacillus brevis 및 Leuconostoc citreum 의 검출법/ 정석호, 발행사항 : 인천, 인하대 대학원, 2002.2
서명 : 필수식품미생물학, 저자 : 민경찬, 출판사 : 광문각 Page : 128 ~ 129 발췌
