PCR

목차

1. Title
2. Object
3. Introduction
4. Material & Methods
5. Result
6. Discussion
7. Further study
7. Reference

본문내용

1. Title : PCR

2. Object
Do selectively amplify wanted region of DNA

3. Introduction

[PCR]

The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

<중 략>

Polymerase Errors
DNA polymerase are not perfect by any means, however they are quite good at what they do. Mistakes and errors do happen in PCR by DNA polymerases in the test tube and in the human body. The polymerases used in PCR often lack 3` to 5` exonuclease activity such as Taq polymerase. This enzyme lacks the ability to correct misincorporated nucleotides, an activity which is present in higher eukaryotes.
A polymerase lacking the 3` to 5` exonuclease activity has a higher error rate, around 1 in 10000 bases are misincorporated.
Recombinant polymerases have been generated and other polymerases have been isolated which are available to more accurate PCR and have a variety of applications such as sequencing.

참고 자료

http://en.wikipedia.org/wiki/Polymerase_chain_reaction
http://kin.naver.com/qna/detail.nhn?d1id=11&dirId=1116&docId=131554969&qb=cGNyIOqysOqzvA==&enc=utf8§ion=kin&rank=5&search_sort=0&spq=0&sp=1&pid=gqORv35Y7uCssb22dK4ssc--366633&sid=TqdUD3tBp04AAG22MX0
http://bric.postech.ac.kr/myboard/read.php?Board=exp_qna&id=41753
http://bric.postech.kr/myboard/read.php?Board=exp_qna&id=13526
http://www.pcrstation.com/pcr-limitations/