HPLC 원리 및 사용법

목차

1. Introduction
2. Classification of HPLCI- Types of HPLC according to the mechanism of separation
3. Choice of separation technique
4. Chromatographic process
5. The advantages of HPLC
6. Instrumentation of HPLC
7. Mobile phase
8. Elution Techniques (Programinig)
9. Isocratic and gradient elution curves
10. Advantages of gradient elution technique
11. PH of mobile phase
12. Effect of buffer used in separationof xanthene alkaloids
13. Mobile Phase Composition Effect on Selectivity
14. / of Mobile phase B (MeOH) and separation selectivity
15. Some solvents used in HPLC and their polarity
16. Treatment of mobile phase
17. B- Pump
18. The main criteria for the pump
19. C- Sample inlet device(Injection port)
20. D- Column
21. Other types of columns used in HPLC
22. E- Detectors (Brain of HPLC)
23. Types of Detectors
24. Applications of HPLC
25. II- Quantitative (assay) and qualitative determination of natural products
26. Quantification of oroidin in Axinella damicornis sponge (assay)
27. Steps of assay
28. Standard curve of oroidin

본문내용

Quantification of compounds by HPLC
Is the process of determination of the unknown concentration of a compound in a known solution.
Identification of compound by HPLC through :
- Comparison of retention time with authentic
- Comparison of UV spectrum of the compound with that of the authentic.
- Comparison of the Mass spectrum with that of the authentic.

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