NMR강의

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Isotopic labeling
As we go to larger proteins (> 10 KDa), things start getting more and more crowded. We start loosing to many residues to overlap, and we cannot assign the whole chain
What we need is more NMR sensitive nuclei in the sample - add a third (and maybe fourth) dimension
To do this we need several things:
a) We need to know the gene (DNA chunk) that is responsible for the synthesis of our proteins
b) We need a molecular biologist to get a plasmid that overexpresses this gene, possibly in an E. coli vector system in minimal media

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