NMR이론발표자료-2

목차

Chapter 4. The NMR Assignment Problem in Biopolymers
1. sequnetial assignments
2. isotope labelling

Chapter 9. Polypeptide Secondary Structures in Proteins by NMR
1. regular secondary structures from observation of short 1H-1H distanses
2. regular secondary structures from spin-spin couplings 3JHNα
3. hydrogen bonds and amide-proton exchange
4. identification of tight turns

본문내용

4.1. sequnetial assignments
* Assignment ?
The aim of the analysis of NMR spectra is to extract all available information about interatomic distances and torsion angles. In the initial stage of investigation by NMR spectroscopy each resonance must be associated with a specific nucleus in the investigated molecule.

General the assignment can be divided in two parts
: The sequential assignment of the amino acids in the protein sequence and the assignment of the amino acid side chains.

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