Stripping probes
- 최초 등록일
- 2014.09.13
- 최종 저작일
- 2014.08
- 4페이지/
한컴오피스
- 가격 1,000원
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목차
1. Introduction
2. Materials
3. Method
4. References
본문내용
Introduction
Southern blotting
DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture.
Membrane
◆ Nitrocellulose membrane
A high quality membrane ideal for blotting of proteins and nucleic acids.
The major features :
☞ Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, fluorescence, or radiolabeling.
☞ Provides high-sensitivity with low background .
◆ Nylon Membrane
Nylon+ is a high quality, 0.45 μm membrane particularly suitable for fast, high-resolution electrotransfer of nucleic acids.
The major features :
☞ High sensitivity for superior detection of nucleic acids.
☞ High binding capacity for nucleic acids.
☞ Intrinsic hydrophobicity for rapid wetting.
Probes
A fragment of DNA used to recognize a specific complementary DNA sequence, or gene(s). Probes can be employed, for example, to bind to the genetic material of microbes for purposes of detection, identification, or, in some cases, inactivation.
참고 자료
http://www.chemicon.com
Molecular Cloning. Third edition. Sambrook/Russell. 2001. p6.57