분자생물학기초실험/ 분자생명공학기초실험 11주차: Preparing Buffer and Separation of Compounds using TLC [A+ 리포트]

소개글

연세대학교 2016년 2학기 A+ 받은 리포트입니다.
실험관련 topic에 대해 많은 조사를 했기에 이 자료 하나로 충분할거에요.

목차

1. Quiz
2. Result
3. Discussion
4. References

본문내용

<Quiz>
1. Explain and compare thin layer chromatography with column chromatography.
Thin Layer Chromatography (TLC) is used to separate the pure individual compounds from a mixture. TLC is one of a quick and inexpensive chromatographic technique that is most widely used to monitor the progress of a reaction by comparing the Rf of a compound with Rf of a known compound.

<Discussion>
In this investigation, many different ratios of eluent systems (mixture of ethyl acetate: hexane) were used to separate and assist the compounds: aniline (A), benzoic acid (B) and triphenylphosphine (C) on the silica gel by capillary action. The main objective of this study was to find appropriate eluent composition that can determine Rf values within 0.3-0.4 for each compound by using TLC. Therefore, our group started with 1:7 (EA: hexane) and found that Rf values for compounds A, B and C were: 0.25, 0.469 and 0.781, respectively.

참고 자료

Basic Molecular Biotechnology Laboratory Booklet – Department of Biotech. Yonsei University.
"Column Chromatography." Column Chromatography. Web. 07 Dec. 2016. <http://www.chemguide.co.uk/analysis/chromatography/column.html>
"Separation of Compounds Using Column Chromatography (Theory): Organic Chemistry Virtual Lab: Chemical Sciences: Amrita Vishwa Vidyapeetham Virtual Lab." Web. 06 Dec. 2016. <http://vlab.amrita.edu/?sub=2&brch=191&sim=341&cnt=1>