분자생물학기초실험/분자생명공학기초실험 3주차: Polymerase Chain Reaction (PCR) and DNA Agarose Gel Electrophoresis [A+ 리포트]
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소개글
연세대학교 2016년 2학기 A+ 받은 리포트입니다.
실험관련 topic에 대해 많은 조사를 했기에 이 자료 하나로 충분할거에요.
목차
<Objective>
<Method>
<Results/Discussion>
<Quiz>
<References>
본문내용
<'Objective> In this investigation, the main objectives are:
① To understand the principle of polymerase chain reaction (PCR) & DNA agarose gel electrophoresis
② To know how to handle the thermocycler machine
③ To know how to analyze the PCR product in DNA agarose gel electrophoresis
<'Method>
Preparation of PCR Mixture (so-called Master Mix)
1. Add 35μL of distilled water (DW) into PCR e-tube
2. Add 5μL of 10x polymerase buffer into the PCR e-tube
3. Add 4μL of 10mM dNTP into the PCR e-tube
참고 자료
A fluorescence resonance energy transfer-based sensor indicates that receptor access to a G protein is unrestricted in a living mammalian cell. Azpiazu I, Gautam N. J Biol Chem. 2004 Jun 25; 279(26):27709-18. Epub 2004 Apr 12. 10.1074/jbc.M403712200 PubMed 15078878
Basic Molecular Biotechnology Laboratory Booklet – Department of Biotech. Yonsei University
"NCBI Reference Sequence: NM_010851.2." Mus Musculus Myeloid Differentiation Primary Response Gene 88 (Myd88), MRNA. NCBI GenBank, Web. 3 Oct. 2016. <https://www.addgene.org/36396/>.
"Preparation of Inserts - Primer Design." Functional Geno-proteome Research Center. Web. 3 Oct. 2016. <http://www.genehunters.co.kr/>.
"Types of Enzymes Involved in DNA Synthesis and Cloning: 7 Types." Biology Discussion. 16 Oct. 2015. Web. 02 Oct. 2016. <http://www.biologydiscussion.com/enzymes/types-of-enzymes-involved-in-dna-synthesis-and-cloning-7-types/12075>.