배아줄기세포의 생산, 분석, 조작 (Establishment, characterization and manipulation of embryonic stem cells)
*경*
다운로드
장바구니
목차
Ⅰ. Establishment of mouse embryonic stem cellⅡ. Establishment of human embryonic stem cell
Ⅲ. Germline chimera
Ⅳ. Characterization of embryonic stem cell
Ⅴ. Genetic manipulation of embryonic stem cell
Reference
본문내용
Ⅰ. Establishment of mouse embryonic stem cellMouse embryonic stem cells were first derived in 1981 (Evans MJ and Kaufman MH, Nature, 1981).
Fig. 1. Inter-relationships of cell lines, teratocarcinomas and embryoid bodies with normal mouse embryos. Arrows indicate routes of cell transfer: a, formation of teratocarcinoma by ectopic implantation of embryos; b, formation of embryoid bodies from teratocarcinoma and vice versa; c, derivation of cell culture from embryoid bodies; d, cell culture obtained directly from solid tumours; e, differntiation to embryoid bodies from culture; f, formation of solid tumours on reinjection of cells from culture; g, transfer of embryonal carcinoma cells either from cell culture or from the core of an embryoid body or from a solid tumour back to a blastocyst. All these procedures may result in chimaerism of the resulting mouse; h, the missing link supplied here.
참고 자료
Evans MJ, Kaufman MH. 1981. Establishment in culture of pluripotential cells from mouse embryos. Nature. 292(5819):154-6.Czechanski A, Byers C, Greenstein I, Schrode N, Donahue LR, Hadjantonakis AK, Reinholdt LG. 2014. Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains. Nat Protoc. 9(3):559-74.
Christine LM, Bernard AJR. 2013. Cloning human embryos. Nature. 498.
Terese Winslow. 2006. Regenerative Medicine.
Wobus AM, Boheler KR. 2005. Embryonic stem cells: prospects for developmental biology and cell therapy. Physiol Rev. 85(2):635-78.
Bradley A, Evans M, Kaufman MH, Robertson E. 1984. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature. 309(5965):255-6.
Martí M, Mulero L, Pardo C, Morera C, Carrió M, Laricchia-Robbio L, Esteban CR, Izpisua Belmonte JC. 2013. Characterization of pluripotent stem cells. Nat Protoc. 8(2):223-53.
Crook JM, Peura TT, Kravets L, Bosman AG, Buzzard JJ, Horne R, Hentze H, Dunn NR, Zweigerdt R, Chua F, Upshall A, Colman A. 2007. The Generation of Six Clinical-Grade Human Embryonic Stem Cell Lines. Cell Stem Cell. 1(5):490-494.
Henson NL, Heaton ML, Holland BH, Hawkins KC, Rawlings BA, Eanes EA, Bozof RG, Powell SW, Grau RE, Fortney JA, Peebles BG, Kumar D, Yoon JI, Godby KN, Collins JA, Sood R, Hixson D, Bowyer FP 3rd, Black AC Jr, Young HE. 2005. Karyotypic analysis of adult pluripotent stem cells. Histol Histopathol. 20(3):769-84.
Myers JD. 1989. Development and application of immunocytochemical staining techniques: a review. Diagn Cytopathol. 5(3):318-30.
Sheridan SD, Surampudi V, Rao RR. 2012. Analysis of embryoid bodies derived from human induced pluripotent stem cells as a means to assess pluripotency. Stem Cells Int. 2012:738910.
Hiyama E, Hiyama K. 2007. Telomere and telomerase in stem cells. Br J Cancer. 96(7):1020-4.
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. 1998. Embryonic stem cell lines derived from human blastocysts. Science. 282(5391):1145-7.
Babinet C, Cohen-Tannoudji M. 2001. Genome engineering via homologous recombination in mouse embryonic stem (ES) cells: an amazingly versatile tool for the study of mammalian biology. An Acad Bras Cienc. 73(3):365-83.