RNA isolation RT-PCR

목차

1. RNA isolation
1) MATERIALS
2) PROCEDURE

2. RT-PCR
1) MATERIALS
2) PROCEDURE

본문내용

1) RNA isolation
MATERIALS
TRIZOL, Chloroform, Isopropanol, 70% Ice-cold EtOH, DEPC-treated Water

PROCEDURE
- remove the media
- Add 500 ul of TRIZOL and pipet the lysate up and down
- Transfer the sample to a e-tube ///////////store at -70°C
- Add 100 ul of chloroform
- Centrifuge the samples for 13000 rpm, 4°C, 15 min
(Separate as: lower- clear pink , interphase- white, upper- clear aqueous)
- Transfer the clear aqueous phase to a new tube
- Add same volume of isopropanol

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