[A+ 식품미생물학실험 영문레포트] Transfer of bacteria (Aseptic technique)

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[A+ 식품미생물학실험 영문레포트]

A+ 평가 받은 식품미생물학실험 영문 레포트입니다.
영문으로 기본적인 내용을 잘 적어놓았기 때문에 영문레포트 작성에 좋은 참고가 될 수 있습니다.

목차

1. Title
2. Date
3. Purpose
4. Materials and Equipment
5. Method
6. Data
7. Result and Discussion
8. Reference

본문내용

5. Method
<Making agar culture medium>
① Suspend 20.0g of the TSA powder in 500ml of purified water in 3 Erlenmeyer flasks each.
② Mix thoroughly with Magnetic bar and Stirrers. and Heat with frequent agitation and boil for few minutes to completely dissolve the powder.
③ Autoclave at 121˚C for 15 minutes.
④ Cool enough to about 50˚C.
⑤ Using a pipette, place liquid agar in each 2 test tube and petri dish. and Close the lids.
＊ When create a slanted agar, test tube should be maintained in the proper angle.
＊ When pour the agar, It should be done within the clean bench.
＊ Be careful not to make bubbles on center of the agar.
＊ If the amount of agar is too small, bacteria can not grow well because of malnutrition.
＊ If after one hour of hardening of the agar, plate upside down to dry because of moisture.

<Inoculating E. coli>
① Sterilize the loop by holding the wire in the flame until it is red-hot.
② While holding the sterile loop and the bacterial culture, remove the cap.

참고 자료

박석기 외 5인, 1995, 최신 미생물학실험, 신광문화사, pp61~74
Naver Encyclopedia http://100.naver.com/