일반생물실험 랩 영어 레포트 5. 유전자 발현 연구 (qRT-PCR)

소개글

"일반생물실험 랩 영어 레포트 5. 유전자 발현 연구 (qRT-PCR)"에 대한 내용입니다.

목차

1. Introduction
2. Materials
3. Methods
4. Results
5. Discussion
6. References

본문내용

Introduction
There are two different qRT-PCR mechanisms. One is a SYBR green dye-based method, while the other is a probe-based method that uses the TaqMan probe. This lab looks at qRT-PCR by the SYBR green dye-based method. SYBR green is a dye which means that unless it is bound to dsDNA, it will produce a much weaker fluorescent signal due to a much weaker excitation of the dye molecules. As amplification proceeds, the amount of DNA product increases, and hence the number of SYBR green molecules incorporated into DNA increases.

참고 자료

Mackay IM; Arden KE; Nitsche A (March 2002). "Real-time PCR in virology". Nucleic Acids Res. 30 (6): 1292–305. .