면역학 SDS-PAGE 실험 레포트

소개글

"면역학 SDS-PAGE 실험 레포트"에 대한 내용입니다.

목차

1.실험제목(title)
2.실험날짜(date):
3.실험목적(purpose):
4.실험 준비물(material-reagent, instrument)
5.실험 방법(method)
6.실험 결과(result)
7.실험 고찰(discussion)
8.참고문헌(reference)

본문내용

· 실험 제목(title): SDS-page와 coomassie blue staining

· 실험날짜(date):

· 실험목적(purpose): sds-page를 통해 원하는 단백질의 유무를 확인하고 marker와 비교하여 분자량을 산출해내기 위함이다.

· 실험 준비물(material-reagent, instrument)
pipet, tip, 5X sample buffer, coomassie blue, whole protein sample, IP sample(BSA, actin), SDS-page gel, 1X TGS buffer, protein marker, rocker, destaining solution, SDS gel electrophoresis, FBS, PBS

참고 자료

Mireille Chevallet, Sylvie Luche, and Thierry Rabilloud, Silver staining of proteins in polyacrylamide gels, Nat Protoc. 2006; 1(4): 1852–1858
약품생화학분과회, 약품생화학실험, 신일북스, 2-8p
https://www.biocompare.com/23650-TrisGlycine-SDS-Buffers/890647-TrisGlycineSDS-1X-Solution-Electrophoresis/
http://www.dynebio.co.kr/yc/bbs/board.php?bo_table=data1&wr_id=14&page=2
http://www.dynebio.co.kr/yc/bbs/board.php?bo_table=data1&wr_id=7&page=3
http://www.komabiotech.co.kr/www/board/board_viewing.phtml?no=502&page=79&type=tboard_board1
https://en.wikipedia.org/wiki/Bromophenol_blue
https://en.wikipedia.org/wiki/SDS-PAGE
https://en.wikipedia.org/wiki/2-Mercaptoethanol
https://en.wikipedia.org/wiki/Polyacrylamide_gel_electrophoresis
https://www.thermofisher.com/order/catalog/product/20278
http://www.berthold-jp.com/pdf/coomassie_blue.pdf
https://en.wikipedia.org/wiki/Bovine_serum_albumin
http://www.komabiotech.co.kr/www/product/productdesc.phtml?seq=92
http://proteomics.rockefeller.edu/ms_silverStaining