[유전공학]제조합 단백질 생산을 위한 host cell
- 최초 등록일
- 2006.04.22
- 최종 저작일
- 2006.04
- 2페이지/
한컴오피스
- 가격 1,000원
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소개글
제조합 단백질 생산을 위한 host cell...숙주세포로 사용할 수 있는 것들의 종류와 그 특징, post translational system의 장단점에 대한 내용
목차
General problems with the production of recombinant protein in E. coli
Production of recombinant protein by eukaryotic cells
본문내용
1. Problems resulting from the sequence of the foreign gene
① foreign gene might contain introns
② foreign gene might contain termination signal ⇒ premature termination
③ foreign gene might contain unfavored codons
2. Problems caused by E. coli
① might not process the recombinant protein correctly e.g. glycosylation
② might not fold the recombinant protein correctly e.g. disulfide bond, inclusion body
③ might degrade the recombinant protein
Production of recombinant protein by eukaryotic cells
1. Recombinant protein from yeast and filamentous fungi
Saccharomyces cerevisiae
① high yield
② unable to glycosylate animal proteins correctly, adding to many sugar unit
③ lacks an efficient secreting system
④ accepted as a safe organism for production of protein for use in medicines, foods
참고 자료
gene cloning