[생물]E.Coli에서의 DNA 추출 과정

소개글

대장균에서의 DNA추출과정이며 영문과 한글 모두로 작성되어 있습니다.

목차

1. Materials
2. Methods

본문내용

2. Methods
① Incubate the E. coli culture overnight at 37℃ with vigorous shaking.
② Pour 1.5 ml of the culture into a microfuge tube. Centrifuge at 12,000 g for 1~2 minutes.
③ Remove the medium, leaving the bacterial pellet as dry as possible.
④ Resuspend the bacterial pellets in 100 µl of ice-cold Solution I by vigorous vortexing.
⑤ Add 200 µl of freshly prepared Solution II
⑥ Add 150 µl of ice-cold Solution III
⑦ Centrifuge at 12,000 g for 5minutes at 4℃ in a microfuge. Transfer the supernatant to a fresh tube.
⑧ Precipitate the double-stranded DNA with 2 volumes of ethanol at room temperature. Mix by vortexing. Allow the mixture to stand for 2 minutes at room temperature.
⑨ Centrifuge at 12,000 g for 5 minutes at 4℃ in a microfuge.
⑩ Remove the supernatant carefully using 1,000 µl micropipette.Stand the tube in an inverted position on a paper towel to allow all of the fluid to drain away.

참고 자료

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