Cellbiocontrol Laboratory(Extracellular matrix effects on neurosphere cell motility)
- 최초 등록일
- 2008.01.20
- 최종 저작일
- 2008.01
- 10페이지/
MS 파워포인트
- 가격 1,000원
소개글
Cellbiocontrol Laboratory(Extracellular matrix effects on neurosphere cell motility) 에 관하여 작성하였습니다.
목차
Introduction
Material & Method
Result
Discussion
본문내용
Introduction
ECM of the central nervous system is responsible for regulatory function
Signals : Cell growth
Differentiation
Migration
Development of CNS organization
There is little information as to the ECM regulation of neural stem cell population
This study examined the behavior cerebellar-derived neurospheres on the matrix component laminin, fibronectin, chondroitin sulfate proteoglycan(CSPG)
Extracellular matrix
(ECM)
“A leader in the cell control”
Cellbiocontrol Laboratory
A)A primary culture of proliferating cells
in monolayer culture
B) Budding neurosphere showed nestin
cells (red) at the circumference
Neurosphere cell
Neural stem cells can be grown in vitro, as either monolayer cultures on an adhesive substrate or as free floating spherical aggregates termed Neurospheres
These in vitro propagated NSCs can proliferate in culture while retaining the potency to differentiate into neurons, oligodendrocytes and glia.
“A leader in the cell control”
Cellbiocontrol Laboratory
Journal Seminar 2004. 6. 22
Material & Method
Cell Culture
Matrices Test
Cell were isolated from postnatal day 3 to 5 C57BL/6 mice pups. Tissue was trysininzed and titurated into a single-cell suspension. The cell were plated overnight in tissue culture flask in N2 media with 5%FBS. Nonadherent cell were aspirated, centrifuged, and replated on an antiadhesive coated flask with N2, 5%FBS, and 10g/ml FGF-2 and EGF to generate in vitro neurosphere.
Journal Seminar 2004. 6. 22
참고 자료
없음
