Separation of pigments by thin _badtags chromatography and their identification using spectroscopy. TLC 법을 이용한 식물 조직의 색소 분리 및 분광분석법
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소개글
plant의 잎을 질소로 얼린다음 갈아서 그 색소에 대해 크로마토그래피하여 분리한 후 이를 분석한 report입니다목차
ABSTRACT : 이번 실험에 대한 요약이 들어있습니다.Key words : 이번 실험에서 중요한 단어들이 수록되어 있습니다.
INTRODUCTION : TLC 법과 Rf의 공식 및 Spectrophotometry에 대해 소개했습니다.
MATERIALS & SUPPLIES : 실험에서 사용한 기구 및 시약을 기록했습니다.
RESULT : 이번 실험에 대한 결과입니다.
DISCUSSION : 실험에 대한 고찰입니다.
REFERENCE : 참조 자료를 기록해 놓았습니다.
본문내용
INTRODUCTIONThe most active photosynthetic tissue in higher plants is the mesophyll of leaves. Mesophyll cells have many chloroplasts, which contain the specialized light-absorbing green pigments, the chlorophylls(1). So, plant is greeny. This experiment is extracting pigment of plant and separated pigment by TLC. And identify using spectrophotometer.
What is TLC and spectrophotometer?
Thin layer chromatography(TLC)
TLC is one of chromatography techniques. Chromatography is widely used for the separation, identification, and determination of the chemical components in complex mixtures. This technique is in which the components of a mixture are separated based upon the rates at which they are carried through a stationary phase by a gaseous or liquid mobile phase.
Chromatographic methods are of two types. In column chromatography, the stationary phase is held in a narrow tube, and the mobile phase is forced though the tube under pressure or by gravity. In planar chromatography, the stationary phase is supported on a flat plate or in the pores of a paper. Here the mobile phase moves through the stationary phase by capillary action or under the influence of gravity(2).
let us now look at the place of TLC among chromatographic techniques. The following is general classification in relation to the nature of the mobile and stationary phases.
참고 자료
1. Lincoln Taiz & Eduardo Zeiger. Photosynthesis: The light reactions. In: Plant physiology 2th edition. Sinauer Associates. 1998; p155-191.2. Douglas A. Skoog, Donald M. West, F.James Holler. Fundamentals of analytical chemistry 6th edition. Saunders college publishing 1992; p535-737.
3. Nelu Grinberg. Modern thin-layer chromatography. Chromatographic science series. 1990; Vol 1, 1-3
4. Keith wilson&John Walker. Chromatographic techniques. In: Principles and Techniques of biochemistry and molecular biology 6th edition. Cambridge 2005; p485-550
5. Kim, Jung Hyun. Seeded emulsion polymerization of methyl methacrylate using aqueous polyurethane dispersion: effect of hard segment on grafting efficiency. Colloids & Surfaces A: Phys. Eng. Asp.; Jul2002, Vol. 207 Issue 1-3, p169, 8p
6. Li, Shao-nan. Relationship between mobility factors (Rf) of two hydrophobic termiticides and selected field and artificial soil parameters. Science of the Total Environment; Dec2007, Vol. 388 Issue 1-3, p206-213, 8p
7. http://www.