SDS-PAGE & Western Blotting Antibody production
- 최초 등록일
- 2008.12.29
- 최종 저작일
- 2008.10
- 9페이지/
한컴오피스
- 가격 1,000원
![할인쿠폰받기](/images/v4/document/ico_det_coupon.gif)
소개글
SDS-PAGE & Western Blotting Antibody production 자료 한글과영문포함
실험레포트나, 자료로 이용하세요
목차
SDS-PAGE,
Stacking gel
Buffer systems
Western blotting의 3단계
#reference
본문내용
SDS-PAGE,
sodium dodecyl sulfate polyacrylamide gelelectrophoresis, is a technique widely used in biochemistry, forensics, genetics and molecular biology to separate proteins according to their electrophoretic mobility (a function of length of polypeptide chain or molecular weight as well as higher order protein folding, posttranslational modifications and other factors).The SDS gel electrophoresis of samples having identical charge to mass ratios results in fractionation by size and is probably the world`s most widely used biochemical method.
Procedure
The solution of proteins to be analyzed is first mixed with SDS, an anionic detergent which denatures secondary and non–disulfide–linked tertiary structures, and applies a negative charge to each protein in proportion to its mass.
isoelectric point and molecular weight particular to its primary structure. This is known as Native PAGE. Adding SDS solves this problem, as it binds to and unfolds the protein, giving a near uniform negative charge along the length of the polypeptide.
참고 자료
http://kin.naver.com/detail/detail.php?d1id=11&dir_id=110205&eid=bdZ0nZ6ImChs2zKAKOGPrug4wqwWqzfu&qb=U0RTLVBBR0U=&pid=ffdPasoi5ThssbtGxoZsss--059635&sid=SSQeFJbVI0kAAGB4xVs
http://cafe.naver.com/kjh791215.cafe?iframe_url=/ArticleRead.nhn%3Farticleid=3453
http://kin.naver.com/detail/detail.php?d1id=11&dir_id=110205&eid=iE6ImnDapxJjyZ+WlFd4FO01nFpdSgeW&qb=U0RTLVBBR0U=&pid=ffdPasoi5ThssbtGxoZsss--059635&sid=SSQeFJbVI0kAAGB4xVs
http://en.wikipedia.org/wiki/SDS-PAGE
http://wwwold.ajou.ac.kr/~biotech/study/western/western%20blot.htm
http://blog.naver.com/umyfriends?Redirect=Log&logNo
=60033512854
http://en.wikipedia.org/wiki/Main_Page
유전자 클로닝 입문 T.A Brown 월드사이언스