brainslice를 wash 및 antibody staining하였다. 12주차엔 DAPI staining, mounting 후 confocal microscope으로 sample을 ... Slide glass에 PBS를 적당히 뿌리고, brainslice를 위에 올린다. Brainslice를 편 뒤, PBS를 pipette 및 휴지로 제거한다. ... Incubation이 끝나면, well 5개에 PBS를 넣어 brainslice를 washing한다.
The brainslice was stained according to the regions through the method of H&E staining. ... However, the problem was the after staining the brainslice Eosin Y we cannot return to Hematoxylin staining ... Therefore, I couldn’t just unfold the brainslice to reconduct Hematoxylin staining, but had to reconduct
Frontal Bone Parietal Bone Occipital Bone Bregma LambdaTTC-stained brainslices Striatum Cerebral cortex ... 2,3,5-triphenyltetrazolium chloride TTC stained normal areas of brain deep red but did not stain infarcted ... 20 min. 3) Place the slices on the paper and then take pictures. 4) Calculate infarct volume and edema