: oppositely charged solute molecules C : displacement of the counter-ionRG-C+ + S+ RG-S++C+RG+C- ... Reversible binding of charged molecular Manipulating by solution pH Dependent on the surface area of ... level of purification Easily scale-up Low costTheory of Ion ExchangeBasis for Ion-Exchange separation
groups R : some support matrix S : oppositely charged solute molecules C : displacement of the counter-ion ... Basis for Ion-Exchange separation Reversible binding of charged molecular Manipulating by solution pH ... Designed as polysaccharides Hydrophilic - ( Sephadex ) Sephadex -25, 50 (50 type is better suited for large